CompactDry VP Vibrio Parahaemolyticus – Shimadzu

Model: VP
Code: 06749
Manufacturer: Shimadzu – Japan

Operating Procedure

1. Compact Dry Plate Preparation

• Open the aluminum storage pouch.
• Remove one set of 4 plates or the required number of plates (1–3 plates); keep the remainder in the sealed pouch to protect from light and humidity.
• Record all necessary information in the note section on the plate.

2. Sample Preparation

• Diluent: Dissolve 7,650g NaCl, 0,724g Na₂HPO₄ và 0,210g KH₂PO₄ in 1L distilled water. Adjust pH to 7,4 using NaOH 1N. Sterilize by autoclaving at 121°C trong 15 minutes.

• Sample Dilution:

  • Take 50g of sample, add 450ml of diluent → homogenize using a stomacher for 1 minute to obtain a 1:10 dilution.

  • Transfer 1ml of the 1:10 dilution into 9ml of diluent and mix thoroughly to obtain a 1:100 dilution.

3. Inoculation

• Use a pipette to aspirate 1ml of the diluted sample.
• Open the Compact Dry plate cap.
• Dispense 1ml of the sample onto the center of the plate surface.
• Close the cap.

4. Incubation

• Invert the capped plate and place it in the incubator.
• Incubate for 20-24 hours at 35°C..

Reading Results:

• Observe and count colonies from the underside of the plate.
• Select plates with a colony count between 10-150 to ensure accuracy.
• Blue or blue-green colonies are the target organisms for identification.

Notes:

• When the colony count exceeds 300 CFU, the entire plate surface may turn blue, making it impossible to distinguish individual colonies. In this case, sample dilution is required before retesting.
• After reading results, plates must be sterilized by autoclaving (121°C. for 30min) prior to disposal.