CompactDry YMR Yeast and Mold Rapid - Shimadzu

CompactDry YMR Yeast and Mold Rapid - Shimadzu

Model: YMR
Code: 54063
Manufacturer: Shimadzu – Janpan

Operating Procedure

• Sample Preparation: Aseptically weigh 10.0g of sample or pipette 10.0ml (for liquid samples) into a sterile container (e.g., sterile bag, dilution bottle, or Whirl-Pak bag).
• Dilution & Homogenization: Add 90.0ml of diluent to achieve a 1:10 ratio, then homogenize. Adjust the pH if necessary. Common diluents include Butterfield’s Phosphate-Buffered Diluent, Maximum Recovery Diluent (MRD), or other suitable solutions per BAM guidelines.
• Serial Dilution: If required, perform further dilutions to reach the appropriate concentration.
• Inoculation: Open the lid of the Compact Dry Yeast and Mold Rapid plate. Use a micropipette to dispense 1.0ml of the diluted sample into the center. The sample will diffuse automatically. Close the lid.

• Incubation: Invert the plates and incubate under the following recognized standards:

  • AOAC: 25 ± 2°C for 48–72 hours.

  • MicroVal / NordVal: 25 ± 1°C for approximately 3 days.

Interpretation of Results

To ensure statistical reliability, select plates with a colony count between 10–150.

• Yeast: Colonies typically appear blue, white, or opaque white.
• Mold: Colonies present a filamentous (fuzzy) appearance with colors characteristic of the specific mold species.

Notes:

• Color Variation: In certain cases, yeast colonies may not exhibit a blue color.
• Selectivity: The Compact Dry medium contains antibiotics to inhibit bacterial growth.
• Overgrowth: If the count exceeds 300 CFU, the entire surface may turn green or blue. In this event, the sample must be further diluted and retested.
• Disposal: After recording results, sterilize the plates via autoclaving (121°C for 30 minutes) before disposal.